Coding

Part:BBa_K2952005:Design

Designed by: Charlotte von Koppenfels   Group: iGEM19_Edinburgh_UG   (2019-10-04)


HydEF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 354
    Illegal XhoI site found at 3016
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 958
    Illegal NgoMIV site found at 1385
    Illegal NgoMIV site found at 1555
    Illegal NgoMIV site found at 2321
    Illegal AgeI site found at 1753
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RFC10 and TypeIIS compatible. Codon usage improved for expression in Rhodobacter sphaeroides, based on https://parts.igem.org/Part:BBa_K2300003#Overview.


Source

genomic sequence, Chlamydomonas reinhardtii

References